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CTL Laboratories - Contract Immune Monitoring and Research White Papers

CTL Laboratories - Contract Immune Monitoring and Research

Cellular Technology Limited: A multicolor Natural Killer cell-mediated cytotoxicity detection A multicolor Natural Killer cell-mediated cytotoxicity detection using fluorescence and direct cell imaging.
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User vs. Software-Dependent Variability of ELISPOT Counts Obtained from ten Different Laboratories In ELISPOT assays, cytokine spots produced by antigen-specific T cells show a broad spectrum of sizes and densities over variable background. Therefore, even experienced investigators are likely to come up with different spot counts when subjectively judging the minimal spot size/density to be counted and the maximal spot size for the cut off between singlecell-derived spots vs. those created by cell clusters. This study aims to find out whether statistics-based automated gating can harmonize spot counts obtained in different laboratories.
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ELISPOT Assays Provide Reproducible Results Among Different Laboratories for T-cell Immune Monitoring - Even in Hands of ELISPOT's Inexperienced Investigators Measurements of antibodies in bodily fluids (e.g., by ELISA) have provided robust and reproducible results for decades and such assays have been validated for monitoring of B-cell immunity. In contrast, measuring T-cell immunity has proven to be a challenge due to the need to test live cells in functional assays ex vivo. Several previous efforts looking into the reproducibility of ex vivo T-cell assays between different laboratories, or even within the same laboratory, have provided rather discouraging results.
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How Frequently are Predicted Peptides Actually Recognised by CD8 Cells? Detection of antigen-specific CD8 cells relies on the use of peptides that can bind to HLA-Class I molecules. There is extensive knowledge on individual HLA-alleles’ peptide binding requirements and for many antigens immunogenic peptides have been defined. The 32 individual peptides that comprise the CEF peptide pool represent such well-defined peptide determinants for Cytomegalo, Epstein Barr, and Flu virus. We tested 42 healthy human donors on the accuracy of these peptide predictions.
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Four-Colour B Cell ELISPOT Assay for Simultaneous Detection of all Four Human IgG Subclasses ELISPOT assays are a key research tool for enumerating antigen-specific B cells.
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Publications by Theme For over 15 years, Prof. Lehmann and the team of scientists working with him have been focused on establishing the scientific foundations of ELISPOT assays and to test the utility of the technology in various fields of T cell diagnostics. CTL’s peer-reviewed publications are listed here by theme with the hopes of providing a useful reference for your research.
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Characterisation of the CMV-Specific CD4 Cell Response Most humans become infected with HCMV. Typically, the immune system controls the infection, but the virus persists and can reactivate in states of immunodeficiency. While the HCMV-specific CD8 cell and antibody response has been accessible to investigation, studies of the CD4 cell subsets have been limited by the low frequency of the antigen-specific CD4 cells in PBMC.
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Publications by Theme Cellular Technology Limited is the world's foremost authority in ELISPOT technology. We have elevated ELISPOT analysis to an exact science through a collection of over 150 peer-reviewed publications.
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Contract Immune Monitoring and Research Establishing new bioanalytical laboratory assays requires a major investment of effort and capital. Would you like to save both time and money? Let CTL's team of experts handle the details for you.
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